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acrylamide gradient sds page gels  (Bio-Rad)


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    Bio-Rad acrylamide gradient sds page gels
    Acrylamide Gradient Sds Page Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 9793 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acrylamide+gradient+sds+page+gels/Acrylamide/pm41496364-79-11-23
    Average 97 stars, based on 9793 article reviews
    acrylamide gradient sds page gels - by Bioz Stars, 2026-10
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    SDS Page:

    Article Title: Direct real-time observation of E- and P-selectin-mediated rolling on cutaneous lymphocyte-associated antigen immobilized on Western blots.
    Article Snippet: Protein concentrations were determined using Bradford’s reagent (Bio-Rad, Hercules, CA). .. Samples representing 25–100 g of protein were electrophoresed through 4–20% acrylamide gradient SDS-PAGE gels (Criterion; Bio-Rad) under standard reducing conditions (5% 2-ME sample buffer). .. Prestained molecular mass standards (Amersham Biosciences, Piscataway, NJ) were included in adjacent lanes in all experiments.

    Article Title: MLP and CARP are linked to chronic PKCα signalling in dilated cardiomyopathy
    Article Snippet: .. Normalized total protein extracts were run on uniform 15% acrylamide, 10% acrylamide, or 4–20% acrylamide gradient SDS-PAGE gels (BioRad, Invitrogen), followed by immunoblotting on nitrocellulose membranes (BioRad) using wet blot technology. .. The nitrocellulose membranes were stained with Ponceau Red, blocked with 5% non fat dry milk (Sainsbury's) or 3% Bovine Serum Albumin (Sigma) in Low Salt Buffer (0.9% NaCl, 9 mM Tris pH 7.4, 0.1% Tween-20) and sequential incubation with the appropriate primary and secondary antibodies with intermittent washing in Low Salt Buffer was performed.

    Article Title: Varicella-zoster virus early infection but not complete replication is required for the induction of chronic hypersensitivity in rat models of postherpetic neuralgia
    Article Snippet: .. Samples were briefly probe-sonicated, heated to 95°C for 5 min, then loaded onto precast 4–15% acrylamide gradient SDS-PAGE gels (Bio-Rad Criterion) and run at 65V until completion. .. Proteins were transferred by electrophoresis to a polyvinylidene difluoride membrane (Millipore Immobolin-FL 00010) overnight at 15V, and membranes blocked overnight at 4°C using LI-COR “Intercept” Blocking Buffer.

    Article Title: Ultramicronized palmitoylethanolamide restores astrocyte-neuron metabolic coupling and Klotho/FGF21 signaling in a triple-transgenic mouse model of Alzheimer's disease.
    Article Snippet: Protein concentration was calculated by BCA assay (Thermo Fisher, Waltham, MA USA). .. Thirty micrograms of the proteins were resolved on precast 4–20 % acrylamide gradient SDS-PAGE gels and then transferred to nitrocellulose membranes using TurboBlot (BioRad, Hercules, CA, USA). ..

    Western Blot:

    Article Title: MLP and CARP are linked to chronic PKCα signalling in dilated cardiomyopathy
    Article Snippet: .. Normalized total protein extracts were run on uniform 15% acrylamide, 10% acrylamide, or 4–20% acrylamide gradient SDS-PAGE gels (BioRad, Invitrogen), followed by immunoblotting on nitrocellulose membranes (BioRad) using wet blot technology. .. The nitrocellulose membranes were stained with Ponceau Red, blocked with 5% non fat dry milk (Sainsbury's) or 3% Bovine Serum Albumin (Sigma) in Low Salt Buffer (0.9% NaCl, 9 mM Tris pH 7.4, 0.1% Tween-20) and sequential incubation with the appropriate primary and secondary antibodies with intermittent washing in Low Salt Buffer was performed.

    other:

    Article Title: Loss of the transcription factor MAFB limits β-cell derivation from human PSCs
    Article Snippet: Cas9 RNP and the cell solution were then added to a Nucleocuvette (Lonza) and nucleofection was carried out using the setting CA137 on the Amaxa 4D-Nucleofector.



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    (A) Pre-nodal mesenteric lymphatic vessel draining to the mesenteric node and inset of the same pre-nodal lymphatic vessel following peri-lymphatic fat removal. (B) Pipette tip (2–4 μm) showing the collected lymph fluid from the pre-nodal lymphatic vessel. (C) Pre-nodal cervical afferent lymphatic vessel draining to the deep cervical node. (D) Detail of the cannulation of a pre-nodal lymphatic vessel. (E) Separation of the mesenteric and cervical lymph proteome (3 μg of protein) on a silver-stained 4%–20% gradient <t>acrylamide</t> <t>SDS-PAGE.</t> (F) Deep Venn area proportional diagram displaying the degree of overlap and differential expression profiling of proteins identified in the mesenteric and cervical lymph using a combination of LFQ proteomics platforms (see protocol). (G) Volcano plot depicting the significant differential expression ( n = 4, p < 0.05 by t test) of 2,752 proteins. Highlighted in blue and red are the 681 proteins showing at least 2-fold downregulation and 799 proteins showing at least 2-fold upregulation in the cervical vs. mesenteric lymph proteomes. (H) Principal component analysis (PCA), generated by MetaboAnalyst, based on the DIA intensities of the proteins identified in n = 4 biological replicates of afferent mesenteric and cervical lymph. (I–K) LFQ comparative analysis of selected proteins displaying significant differences in their relative abundance (extracted from DIA intensities) as determined in (G). Blue and orange dots depict the mesenteric and cervical lymph proteins respectively. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.05: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (L) Euclidian Ward’s dual heatmap of the top 2,000 proteins identified in all four biological replicates of mouse pre-nodal mesenteric and cervical lymph. The map highlights the significant difference in the proteomic signature of the two biological fluids harvested from the distinct anatomical regions. Only proteins that passed a selected significance statistical threshold (ANOVA/t test applied in PEAKS XPro, p < 0.05) are displayed in the heatmap. (M and N) The subsequently generated METASCAPE analysis of protein networks and GO annotations defining the different molecular functions and metabolic pathways of proteins from mesenteric (M) vs. cervical (N) lymph. All proteins and details of LFQ analysis and GO annotations related to the mesenteric and cervical proteomic analysis are presented in .
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    acrylamide gradient sds page gels  (Bio-Rad)
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    (A) Pre-nodal mesenteric lymphatic vessel draining to the mesenteric node and inset of the same pre-nodal lymphatic vessel following peri-lymphatic fat removal. (B) Pipette tip (2–4 μm) showing the collected lymph fluid from the pre-nodal lymphatic vessel. (C) Pre-nodal cervical afferent lymphatic vessel draining to the deep cervical node. (D) Detail of the cannulation of a pre-nodal lymphatic vessel. (E) Separation of the mesenteric and cervical lymph proteome (3 μg of protein) on a silver-stained 4%–20% gradient <t>acrylamide</t> <t>SDS-PAGE.</t> (F) Deep Venn area proportional diagram displaying the degree of overlap and differential expression profiling of proteins identified in the mesenteric and cervical lymph using a combination of LFQ proteomics platforms (see protocol). (G) Volcano plot depicting the significant differential expression ( n = 4, p < 0.05 by t test) of 2,752 proteins. Highlighted in blue and red are the 681 proteins showing at least 2-fold downregulation and 799 proteins showing at least 2-fold upregulation in the cervical vs. mesenteric lymph proteomes. (H) Principal component analysis (PCA), generated by MetaboAnalyst, based on the DIA intensities of the proteins identified in n = 4 biological replicates of afferent mesenteric and cervical lymph. (I–K) LFQ comparative analysis of selected proteins displaying significant differences in their relative abundance (extracted from DIA intensities) as determined in (G). Blue and orange dots depict the mesenteric and cervical lymph proteins respectively. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.05: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (L) Euclidian Ward’s dual heatmap of the top 2,000 proteins identified in all four biological replicates of mouse pre-nodal mesenteric and cervical lymph. The map highlights the significant difference in the proteomic signature of the two biological fluids harvested from the distinct anatomical regions. Only proteins that passed a selected significance statistical threshold (ANOVA/t test applied in PEAKS XPro, p < 0.05) are displayed in the heatmap. (M and N) The subsequently generated METASCAPE analysis of protein networks and GO annotations defining the different molecular functions and metabolic pathways of proteins from mesenteric (M) vs. cervical (N) lymph. All proteins and details of LFQ analysis and GO annotations related to the mesenteric and cervical proteomic analysis are presented in .
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    (A) Pre-nodal mesenteric lymphatic vessel draining to the mesenteric node and inset of the same pre-nodal lymphatic vessel following peri-lymphatic fat removal. (B) Pipette tip (2–4 μm) showing the collected lymph fluid from the pre-nodal lymphatic vessel. (C) Pre-nodal cervical afferent lymphatic vessel draining to the deep cervical node. (D) Detail of the cannulation of a pre-nodal lymphatic vessel. (E) Separation of the mesenteric and cervical lymph proteome (3 μg of protein) on a silver-stained 4%–20% gradient <t>acrylamide</t> <t>SDS-PAGE.</t> (F) Deep Venn area proportional diagram displaying the degree of overlap and differential expression profiling of proteins identified in the mesenteric and cervical lymph using a combination of LFQ proteomics platforms (see protocol). (G) Volcano plot depicting the significant differential expression ( n = 4, p < 0.05 by t test) of 2,752 proteins. Highlighted in blue and red are the 681 proteins showing at least 2-fold downregulation and 799 proteins showing at least 2-fold upregulation in the cervical vs. mesenteric lymph proteomes. (H) Principal component analysis (PCA), generated by MetaboAnalyst, based on the DIA intensities of the proteins identified in n = 4 biological replicates of afferent mesenteric and cervical lymph. (I–K) LFQ comparative analysis of selected proteins displaying significant differences in their relative abundance (extracted from DIA intensities) as determined in (G). Blue and orange dots depict the mesenteric and cervical lymph proteins respectively. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.05: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (L) Euclidian Ward’s dual heatmap of the top 2,000 proteins identified in all four biological replicates of mouse pre-nodal mesenteric and cervical lymph. The map highlights the significant difference in the proteomic signature of the two biological fluids harvested from the distinct anatomical regions. Only proteins that passed a selected significance statistical threshold (ANOVA/t test applied in PEAKS XPro, p < 0.05) are displayed in the heatmap. (M and N) The subsequently generated METASCAPE analysis of protein networks and GO annotations defining the different molecular functions and metabolic pathways of proteins from mesenteric (M) vs. cervical (N) lymph. All proteins and details of LFQ analysis and GO annotations related to the mesenteric and cervical proteomic analysis are presented in .
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    acrylamide gradient sds page gel  (Bio-Rad)
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    (A) Pre-nodal mesenteric lymphatic vessel draining to the mesenteric node and inset of the same pre-nodal lymphatic vessel following peri-lymphatic fat removal. (B) Pipette tip (2–4 μm) showing the collected lymph fluid from the pre-nodal lymphatic vessel. (C) Pre-nodal cervical afferent lymphatic vessel draining to the deep cervical node. (D) Detail of the cannulation of a pre-nodal lymphatic vessel. (E) Separation of the mesenteric and cervical lymph proteome (3 μg of protein) on a silver-stained 4%–20% gradient <t>acrylamide</t> <t>SDS-PAGE.</t> (F) Deep Venn area proportional diagram displaying the degree of overlap and differential expression profiling of proteins identified in the mesenteric and cervical lymph using a combination of LFQ proteomics platforms (see protocol). (G) Volcano plot depicting the significant differential expression ( n = 4, p < 0.05 by t test) of 2,752 proteins. Highlighted in blue and red are the 681 proteins showing at least 2-fold downregulation and 799 proteins showing at least 2-fold upregulation in the cervical vs. mesenteric lymph proteomes. (H) Principal component analysis (PCA), generated by MetaboAnalyst, based on the DIA intensities of the proteins identified in n = 4 biological replicates of afferent mesenteric and cervical lymph. (I–K) LFQ comparative analysis of selected proteins displaying significant differences in their relative abundance (extracted from DIA intensities) as determined in (G). Blue and orange dots depict the mesenteric and cervical lymph proteins respectively. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.05: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (L) Euclidian Ward’s dual heatmap of the top 2,000 proteins identified in all four biological replicates of mouse pre-nodal mesenteric and cervical lymph. The map highlights the significant difference in the proteomic signature of the two biological fluids harvested from the distinct anatomical regions. Only proteins that passed a selected significance statistical threshold (ANOVA/t test applied in PEAKS XPro, p < 0.05) are displayed in the heatmap. (M and N) The subsequently generated METASCAPE analysis of protein networks and GO annotations defining the different molecular functions and metabolic pathways of proteins from mesenteric (M) vs. cervical (N) lymph. All proteins and details of LFQ analysis and GO annotations related to the mesenteric and cervical proteomic analysis are presented in .
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    (A) Pre-nodal mesenteric lymphatic vessel draining to the mesenteric node and inset of the same pre-nodal lymphatic vessel following peri-lymphatic fat removal. (B) Pipette tip (2–4 μm) showing the collected lymph fluid from the pre-nodal lymphatic vessel. (C) Pre-nodal cervical afferent lymphatic vessel draining to the deep cervical node. (D) Detail of the cannulation of a pre-nodal lymphatic vessel. (E) Separation of the mesenteric and cervical lymph proteome (3 μg of protein) on a silver-stained 4%–20% gradient <t>acrylamide</t> <t>SDS-PAGE.</t> (F) Deep Venn area proportional diagram displaying the degree of overlap and differential expression profiling of proteins identified in the mesenteric and cervical lymph using a combination of LFQ proteomics platforms (see protocol). (G) Volcano plot depicting the significant differential expression ( n = 4, p < 0.05 by t test) of 2,752 proteins. Highlighted in blue and red are the 681 proteins showing at least 2-fold downregulation and 799 proteins showing at least 2-fold upregulation in the cervical vs. mesenteric lymph proteomes. (H) Principal component analysis (PCA), generated by MetaboAnalyst, based on the DIA intensities of the proteins identified in n = 4 biological replicates of afferent mesenteric and cervical lymph. (I–K) LFQ comparative analysis of selected proteins displaying significant differences in their relative abundance (extracted from DIA intensities) as determined in (G). Blue and orange dots depict the mesenteric and cervical lymph proteins respectively. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.05: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (L) Euclidian Ward’s dual heatmap of the top 2,000 proteins identified in all four biological replicates of mouse pre-nodal mesenteric and cervical lymph. The map highlights the significant difference in the proteomic signature of the two biological fluids harvested from the distinct anatomical regions. Only proteins that passed a selected significance statistical threshold (ANOVA/t test applied in PEAKS XPro, p < 0.05) are displayed in the heatmap. (M and N) The subsequently generated METASCAPE analysis of protein networks and GO annotations defining the different molecular functions and metabolic pathways of proteins from mesenteric (M) vs. cervical (N) lymph. All proteins and details of LFQ analysis and GO annotations related to the mesenteric and cervical proteomic analysis are presented in .
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    hand casted acrylamide sds page gradient gel  (Bio-Rad)
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    Bio-Rad hand casted acrylamide sds page gradient gel
    (A) Pre-nodal mesenteric lymphatic vessel draining to the mesenteric node and inset of the same pre-nodal lymphatic vessel following peri-lymphatic fat removal. (B) Pipette tip (2–4 μm) showing the collected lymph fluid from the pre-nodal lymphatic vessel. (C) Pre-nodal cervical afferent lymphatic vessel draining to the deep cervical node. (D) Detail of the cannulation of a pre-nodal lymphatic vessel. (E) Separation of the mesenteric and cervical lymph proteome (3 μg of protein) on a silver-stained 4%–20% gradient <t>acrylamide</t> <t>SDS-PAGE.</t> (F) Deep Venn area proportional diagram displaying the degree of overlap and differential expression profiling of proteins identified in the mesenteric and cervical lymph using a combination of LFQ proteomics platforms (see protocol). (G) Volcano plot depicting the significant differential expression ( n = 4, p < 0.05 by t test) of 2,752 proteins. Highlighted in blue and red are the 681 proteins showing at least 2-fold downregulation and 799 proteins showing at least 2-fold upregulation in the cervical vs. mesenteric lymph proteomes. (H) Principal component analysis (PCA), generated by MetaboAnalyst, based on the DIA intensities of the proteins identified in n = 4 biological replicates of afferent mesenteric and cervical lymph. (I–K) LFQ comparative analysis of selected proteins displaying significant differences in their relative abundance (extracted from DIA intensities) as determined in (G). Blue and orange dots depict the mesenteric and cervical lymph proteins respectively. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.05: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (L) Euclidian Ward’s dual heatmap of the top 2,000 proteins identified in all four biological replicates of mouse pre-nodal mesenteric and cervical lymph. The map highlights the significant difference in the proteomic signature of the two biological fluids harvested from the distinct anatomical regions. Only proteins that passed a selected significance statistical threshold (ANOVA/t test applied in PEAKS XPro, p < 0.05) are displayed in the heatmap. (M and N) The subsequently generated METASCAPE analysis of protein networks and GO annotations defining the different molecular functions and metabolic pathways of proteins from mesenteric (M) vs. cervical (N) lymph. All proteins and details of LFQ analysis and GO annotations related to the mesenteric and cervical proteomic analysis are presented in .
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    (A) Pre-nodal mesenteric lymphatic vessel draining to the mesenteric node and inset of the same pre-nodal lymphatic vessel following peri-lymphatic fat removal. (B) Pipette tip (2–4 μm) showing the collected lymph fluid from the pre-nodal lymphatic vessel. (C) Pre-nodal cervical afferent lymphatic vessel draining to the deep cervical node. (D) Detail of the cannulation of a pre-nodal lymphatic vessel. (E) Separation of the mesenteric and cervical lymph proteome (3 μg of protein) on a silver-stained 4%–20% gradient acrylamide SDS-PAGE. (F) Deep Venn area proportional diagram displaying the degree of overlap and differential expression profiling of proteins identified in the mesenteric and cervical lymph using a combination of LFQ proteomics platforms (see protocol). (G) Volcano plot depicting the significant differential expression ( n = 4, p < 0.05 by t test) of 2,752 proteins. Highlighted in blue and red are the 681 proteins showing at least 2-fold downregulation and 799 proteins showing at least 2-fold upregulation in the cervical vs. mesenteric lymph proteomes. (H) Principal component analysis (PCA), generated by MetaboAnalyst, based on the DIA intensities of the proteins identified in n = 4 biological replicates of afferent mesenteric and cervical lymph. (I–K) LFQ comparative analysis of selected proteins displaying significant differences in their relative abundance (extracted from DIA intensities) as determined in (G). Blue and orange dots depict the mesenteric and cervical lymph proteins respectively. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.05: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (L) Euclidian Ward’s dual heatmap of the top 2,000 proteins identified in all four biological replicates of mouse pre-nodal mesenteric and cervical lymph. The map highlights the significant difference in the proteomic signature of the two biological fluids harvested from the distinct anatomical regions. Only proteins that passed a selected significance statistical threshold (ANOVA/t test applied in PEAKS XPro, p < 0.05) are displayed in the heatmap. (M and N) The subsequently generated METASCAPE analysis of protein networks and GO annotations defining the different molecular functions and metabolic pathways of proteins from mesenteric (M) vs. cervical (N) lymph. All proteins and details of LFQ analysis and GO annotations related to the mesenteric and cervical proteomic analysis are presented in .

    Journal: Cell reports

    Article Title: Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load

    doi: 10.1016/j.celrep.2024.114311

    Figure Lengend Snippet: (A) Pre-nodal mesenteric lymphatic vessel draining to the mesenteric node and inset of the same pre-nodal lymphatic vessel following peri-lymphatic fat removal. (B) Pipette tip (2–4 μm) showing the collected lymph fluid from the pre-nodal lymphatic vessel. (C) Pre-nodal cervical afferent lymphatic vessel draining to the deep cervical node. (D) Detail of the cannulation of a pre-nodal lymphatic vessel. (E) Separation of the mesenteric and cervical lymph proteome (3 μg of protein) on a silver-stained 4%–20% gradient acrylamide SDS-PAGE. (F) Deep Venn area proportional diagram displaying the degree of overlap and differential expression profiling of proteins identified in the mesenteric and cervical lymph using a combination of LFQ proteomics platforms (see protocol). (G) Volcano plot depicting the significant differential expression ( n = 4, p < 0.05 by t test) of 2,752 proteins. Highlighted in blue and red are the 681 proteins showing at least 2-fold downregulation and 799 proteins showing at least 2-fold upregulation in the cervical vs. mesenteric lymph proteomes. (H) Principal component analysis (PCA), generated by MetaboAnalyst, based on the DIA intensities of the proteins identified in n = 4 biological replicates of afferent mesenteric and cervical lymph. (I–K) LFQ comparative analysis of selected proteins displaying significant differences in their relative abundance (extracted from DIA intensities) as determined in (G). Blue and orange dots depict the mesenteric and cervical lymph proteins respectively. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.05: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (L) Euclidian Ward’s dual heatmap of the top 2,000 proteins identified in all four biological replicates of mouse pre-nodal mesenteric and cervical lymph. The map highlights the significant difference in the proteomic signature of the two biological fluids harvested from the distinct anatomical regions. Only proteins that passed a selected significance statistical threshold (ANOVA/t test applied in PEAKS XPro, p < 0.05) are displayed in the heatmap. (M and N) The subsequently generated METASCAPE analysis of protein networks and GO annotations defining the different molecular functions and metabolic pathways of proteins from mesenteric (M) vs. cervical (N) lymph. All proteins and details of LFQ analysis and GO annotations related to the mesenteric and cervical proteomic analysis are presented in .

    Article Snippet: Lymph proteins (10–20 μg) were mixed with the sample buffer, heated at 95 °C for 5 min, and run on a 4–20% gradient acrylamide SDS (PAGE) gels (Novex, Tris-Glycine Mini Gels, cat# XV04205PK20, from ThermoFisher Scientific) at 160 V constant following the manufacturer protocols.

    Techniques: Transferring, Staining, SDS Page, Quantitative Proteomics, Generated

    Journal: Cell reports

    Article Title: Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load

    doi: 10.1016/j.celrep.2024.114311

    Figure Lengend Snippet:

    Article Snippet: Lymph proteins (10–20 μg) were mixed with the sample buffer, heated at 95 °C for 5 min, and run on a 4–20% gradient acrylamide SDS (PAGE) gels (Novex, Tris-Glycine Mini Gels, cat# XV04205PK20, from ThermoFisher Scientific) at 160 V constant following the manufacturer protocols.

    Techniques: Recombinant, Protease Inhibitor, Western Blot, Bicinchoninic Acid Protein Assay, Saline, Purification, Membrane, Sterility, Control, Software, Microscopy, Mass Spectrometry